Giardiasis
Heat shock protein 90 plays diversified roles in different microbes. Like any other pathogen Giardia lamblia also uses heat shock protein machinery to survive under stress full conditions put forth by host. Mass spectrometric analysis of Hsp90 from G.lamblia, revealed a novel trans-splicing based expression of GlHsp90 in this minimalist protozoan parasite. We have recently shown that Hsp90 in Giardia is arranged as a split gene, HspN and HspC separated by 777 kb intergenic sequence. The full length Hsp90 transcript is a resultant of a novel trans-splicing phenomenon which is mediated by spliceosomal complex. The mature full length transcript has all the hallmarks of the canonical Hsp90. The protein band that corresponded to a region of around 80 kDa was identified to be GlHsp90. Hence we provide evidence for such a reconstruction mechanism at proteomic level. Sequencing of the junctional peptide by MS/MS provides evidence of functional full length Hsp90 in Giardia, a novel approach to identify trans-splicing event using mass spectrometry. (Nageshan RK et al., JBC 2011, Roy N et al., Biochim Biophys Acta 2011)
